Methods

RNA plays a central role in biology, but there is still much to learn about the molecule’s life cycle in the cell. In recent years, scientists have devised methods that can take snapshots of messenger RNAs...

A kink-turn (K-turn) is a three-dimensional RNA structure that exists in all three primary phylogenetic domains. Reseaerchers at Sun Yat-sen University have developed the RIP-PEN-seq method to identify the...

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been difficult in part because a simple method to simultaneously quantify their abundance and chemical modifications is lacking. Researchers at the Centre for Genomic Regulation (CRG), Spain ...

Why do mRNA expression values need to be normalized? The unification of mRNA expression value measurements across studies, or the normalization of mRNA data, is a significant problem in biomedical and life science research. The abundance of transcripts is measured ...

Established prognostic tests based on limited numbers of transcripts can identify high-risk breast cancer patients yet are approved only for...

Characterizing the topology of gene regulatory networks (GRNs) is a fundamental problem in systems biology. The advent of single cell...

RNA-seq followed by de novo transcriptome assembly has been a transformative technique in biological research of non-model organisms, but the computational processing of RNA-seq data entails...

Dimensionality reduction and visualization play an important role in biological data analysis, such as data interpretation of single-cell RNA sequences (scRNA...

RNA-sequencing (RNA-seq) has been widely used to study the dynamic expression patterns of transcribed genes, which can lead to new biological insights. However, processing and analyzing these huge...

RNA velocity provides an approach for inferring cellular state transitions from single-cell RNA sequencing (scRNA-seq) data. Conventional RNA...