Methods

RNA sequencing analysis focus on the detection of differential gene expression changes that meet a two-fold minimum change between groups. The variability present in RNA sequencing data may obscure...

Single-cell RNA sequencing reveals 40 different cell types in Stylophora pistillata, a reef-building stony coral native to the Indo-Pacific oceans. The calcium carbonate skeleton of stony coral colonies are the...

Despite substantial progress in single-cell RNA-seq (scRNA-seq) data analysis methods, there is still little agreement on how to best normalize such data...

In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to the COVID-19 pandemic, was reported. In the face of...

Alternative RNA isoforms are defined by promoter choice, alternative splicing, and polyA site selection. Although differential isoform expression is known to play a large regulatory role in eukaryotes, it has proved challenging to study with standard short-read RNA-seq because of ...

Conventional scRNA-seq expression analyses rely on the availability of a high quality genome annotation. Yet, as researchers show here with scRNA-seq experiments and analyses spanning human, mouse...

A simplistic understanding of the central dogma falls short in correlating the number of genes in the genome to the number of proteins in the proteome.

Glial cells of the central nervous system (CNS), including astrocytes and microglia, play critical roles in development, tissue repair, and homeostasis. However, dysregulated astrocyte and microglia...

Not all cancer cells within a tumor are created equal; nor do all immune cells (or all liver or brain cells) in your body have the same job. Much of their function depends on their location. Now, researchers at...

Next-generation sequencing allows researchers to efficiently determine the sequences of hundreds of millions of short DNA fragments from an experiment. Many experiments use next-generation sequencing...