Methods

Correlations in gene expression are used to infer functional and regulatory relationships between genes. However, correlations are often calculated...

Late prenatal development of the human neocortex encompasses a critical period of cortical expansion and glial development (cells that are essential to the central nervous system), which is poorly understood...

Single-cell transcriptomics technologies allow researchers to investigate how individual cells, in complex multicellular organisms, differentially use their common genomic DNA. In plant biology...

Ribonucleic acids (RNAs) are fundamental molecules that control regulation and expression of the genome and therefore the function of a cell. Robust analysis and quantification of RNA transcripts hold...

Single-cell atlases promise to provide a ‘missing link’ between genes, diseases and therapies. By identifying the specific cell types, states, programs and contexts where disease-implicated genes act...

Alternative splicing (AS) is a post-transcriptional regulation that leads to the complexity of the transcriptome. Despite the growing importance of...

The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has advanced our understanding of the pathogenesis of disease...

Alternative polyadenylation (APA) plays an important role in posttranscriptional gene regulation such as transcript stability and translation efficiency. However, our knowledge about APA dynamics at the single...

Recent advances in single-cell sequencing technologies have provided unprecedented opportunities to measure the gene expression profile and RNA velocity of individual cells. However...

Bulk RNA-sequencing technologies have provided invaluable insights into host and bacterial gene expression and associated regulatory networks. Nevertheless, the majority of these approaches...