Methods

Single-cell RNA-sequencing (scRNA-seq) has become a widely used tool for both basic and translational biomedical research. In scRNA-seq data analysis, cell type annotation is an essential but challenging step...

Large-scale studies of single-cell sequencing and biological experiments have successfully revealed expression patterns that distinguish different cell...

A single-cell resolution atlas of the human musculoskeletal system could be transformative to understanding and treating conditions including arthritis, cancer and fractures. To generate a single...

Infection by coronavirus SARS-CoV2 is a severe and often deadly disease that has implications for the respiratory system and multiple organs across the human body. While the effects in the lung have...

CosMx™ Spatial Molecular Imager (SMI) data is flat-out awe-inspiring: even a perfunctory analysis of a single run produces a terabyte of data, gorgeous images (see below), and spatial relationships...

The growing number of available single-cell gene expression datasets from different species creates opportunities to explore evolutionary relationships between cell types across species. Cross-species integration of single-cell RNA-sequencing data has been particularly informative in this context. However, in order to ...

Single-cell RNA sequencing technology is one of the most cost-effective ways to uncover transcriptomic heterogeneity. With the rapid rise of this technology...

The Human Breast Cell Atlas, recently published in Nature, is a landmark achievement for mammary biology as the world’s most comprehensive atlas of healthy breast tissue. The current “atlas era” of cell biology represents a marked shift in the field. Whereas ...

The increasing amount of data recorded in medical research can only lead to scientific breakthroughs and essential therapies for patients if interpreted...

Differential allele-specific expression (ASE) is a powerful tool to study context-specific cis-regulation of gene expression. Such effects can reflect the interaction between genetic or epigenetic factors...