Protein functional effector sncRNAs (pfeRNAs) are approximately 30-60 nucleotides (nt), of which the extraction method from plasma has not yet been...
Read More »Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
Tissues are complex mixtures of different cell subtypes, and this diversity is increasingly characterized using high-throughput single cell analysis methods. However, these efforts are hindered...
Read More »MagBio Genomics Inc. develops automated RNA purification chemistry
Mawi DNA Technologies, a biotechnology company focusing on the development of innovative technologies for biosampling, announces that MagBio Genomics has successfully developed an automated RNA purification method for iSWAB-RNA v2 on the Thermofisher KingFisher™ Flex platform with its HighPrep™ Total ...
Read More »Microtissue RNA extraction for reproducible and robust spatial transcriptome analysis
Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in a morphological...
Read More »Electrical lysis and RNA extraction from single cells for RNA sequencing
Researchers from the RIKEN Cluster for Pioneering Research have developed a microfluidic method for electrical lysis and RNA extraction from single fixed cells leveraging reversible cross-linker dithiobis(succinimidyl propionate) (DSP). The microfluidic system...
Read More »Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next-generation sequencing
Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes and have recently garnered interest because of their diagnostic and...
Read More »Optimizing exosomal RNA isolation for RNA-Seq analyses of archival sera specimens
Exosomes are endosome-derived membrane vesicles that contain proteins, lipids, and nucleic acids. The exosomal transcriptome mediates intercellular communication, and represents an understudied reservoir of novel biomarkers for human diseases...
Read More »A practical solution for preserving single cells for RNA sequencing
The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material...
Read More »RPAD – High-purity isolation of circular RNA
High-throughput RNA sequencing methods coupled with specialized bioinformatic analyses have recently uncovered tens of thousands of unique circular (circ)RNAs, but their complete sequences, genes of origin and functions are largely unknown. Given that circRNAs lack free ends and are thus ...
Read More »Improved RNA extraction from neurons
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, researchers from the RIKEN Institute present significant improvements to the protocol for isolation ...
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