Our understanding of nerve regeneration can be enhanced by delineating its underlying molecular activities at single-neuron resolution in model organisms such as Caenorhabditis elegans. Existing cell...
Read More »A protocol for single nucleus RNA-seq from frozen skeletal muscle
Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic cells are resistant to single-cell RNA...
Read More »Isolation of high-quality total RNA from small animal articular cartilage for RNA-seq analysis
Researchers from the University of Debrecen highlight the importance of obtaining high-quality RNA for RNA-seq analysis, as poor RNA quality...
Read More »Comprehensive Capture of FFPE RNA for RNA-Seq Using a Continuous Synthesis Chemistry and Post-Library Ribodepletion
The most common archived biospecimen in oncology, formalin-fixed paraffin-embedded (FFPE) tissue is considered a key source material for gene expression profiling of tumors using RNA sequencing (RNA-Seq). However, FFPE RNA is highly degraded and is therefore suboptimal for most currently ...
Read More »DeLTa-Seq – direct-lysate targeted RNA-Seq from crude tissue lysate
Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing...
Read More »A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides
Protein functional effector sncRNAs (pfeRNAs) are approximately 30-60 nucleotides (nt), of which the extraction method from plasma has not yet been...
Read More »Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models
Tissues are complex mixtures of different cell subtypes, and this diversity is increasingly characterized using high-throughput single cell analysis methods. However, these efforts are hindered...
Read More »MagBio Genomics Inc. develops automated RNA purification chemistry
Mawi DNA Technologies, a biotechnology company focusing on the development of innovative technologies for biosampling, announces that MagBio Genomics has successfully developed an automated RNA purification method for iSWAB-RNA v2 on the Thermofisher KingFisher™ Flex platform with its HighPrep™ Total ...
Read More »Microtissue RNA extraction for reproducible and robust spatial transcriptome analysis
Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in a morphological...
Read More »Electrical lysis and RNA extraction from single cells for RNA sequencing
Researchers from the RIKEN Cluster for Pioneering Research have developed a microfluidic method for electrical lysis and RNA extraction from single fixed cells leveraging reversible cross-linker dithiobis(succinimidyl propionate) (DSP). The microfluidic system...
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