Statistical Analysis

How good are those RNA-seq data?

The enormous number of public gene expression data sets can turn into research gold when mined for any number of biological questions. But digging into the data of other researchers is often fraught by a lack of metadata. In particular, ...

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PathwaySeq – Pathway analysis for RNA-Seq data

rna-seq

A variety of pathway/gene-set approaches have been proposed to provide evidence of higher-level biological phenomena in the association of expression with experimental condition or clinical outcome. Among these approaches, it has been repeatedly shown that resampling methods are far preferable ...

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Understanding the differences between microarray and RNA-Seq technologies for measuring gene expression is necessary for informed design of experiments and choice of data analysis methods

rna-seq

Previous comparisons between microarrays and RNA-Seq have come to sometimes contradictory conclusions, which researchers from Princeton University suggest result from a lack of attention to the intensity-dependent nature of variation generated by the technologies. To examine this trend, they carried ...

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