Workflow

The analysis of single cell gene expression across thousands of individual cells within a tissue or microenvironment is a valuable tool for identifying cell composition, discrimination of functional states, and molecular pathways underlying observed tissue...

The neurodegenerative disease ALS causes motor neuron death and paralysis. However, long before the cells die, they lose contact with the muscles as their axons atrophy. Researchers at Karolinska...

Researchers from the Mayo Clinic used single cell RNA-Seq to examine molecular heterogeneity in multiple myeloma (MM) in 597 CD138 positive cells from bone marrow aspirates of 15 patients at different stages of disease progression. 790 genes were...

Researchers from the University of Cambridge have developed a new method to capture protein-RNA complexes which is compatible with downstream proteomics or RNA-Seq. The new method is based on the acid guanidinium thiocyanate-phenol-chloroform (AGPC) which is commonly used to extract ...

Mammalian gene expression is inherently stochastic, and results in discrete bursts of RNA molecules that are synthesized from each allele. Although transcription is known to be regulated by promoters and enhancers, it is unclear how cis-regulatory sequences encode transcriptional burst ...

Droplet microfluidics has revolutionized single-cell RNA sequencing, offering a low-cost, high-throughput method for single-cell genomics. However, this method has been limited in its ability to capture complete RNA transcription information. Researchers at Cornell – led by Iwijn De Vlaminck...

Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and ...

Estonian scientists have announced the invention of a genetic testing technology to analyse the number of clinical biomarkers at the single-molecule level, which enhances the sensitivity of tests in precision medicine and will make them more affordable in future...

Tools allow scientists to understand the activity of naturally occurring microbiomes in response to real-world conditions and diet Researchers from the University of Chicago have developed a...

The production of antibodies by B cells is an important component of adaptive immunity. While antibodies often afford protection against pathogens such as viruses and bacteria, one class of antibodies, known as IgE, is involved in the pathogenesis of allergies ...