The analysis of single cell gene expression across thousands of individual cells within a tissue or microenvironment is a valuable tool for identifying cell composition, discrimination of functional states, and molecular pathways underlying observed tissue...
Read More »New RNA Sequencing method for studying ALS more effectively
The neurodegenerative disease ALS causes motor neuron death and paralysis. However, long before the cells die, they lose contact with the muscles as their axons atrophy. Researchers at Karolinska...
Read More »Molecular signatures of multiple myeloma progression through single cell RNA-Seq
Researchers from the Mayo Clinic used single cell RNA-Seq to examine molecular heterogeneity in multiple myeloma (MM) in 597 CD138 positive cells from bone marrow aspirates of 15 patients at different stages of disease progression. 790 genes were...
Read More »OOPS is a simple method to study RNA-protein interactions
Researchers from the University of Cambridge have developed a new method to capture protein-RNA complexes which is compatible with downstream proteomics or RNA-Seq. The new method is based on the acid guanidinium thiocyanate-phenol-chloroform (AGPC) which is commonly used to extract ...
Read More »Allelic single-cell RNA sequencing is a powerful model for investigating transcriptional kinetics
Mammalian gene expression is inherently stochastic, and results in discrete bursts of RNA molecules that are synthesized from each allele. Although transcription is known to be regulated by promoters and enhancers, it is unclear how cis-regulatory sequences encode transcriptional burst ...
Read More »Elegant trick improves single-cell RNA sequencing
Droplet microfluidics has revolutionized single-cell RNA sequencing, offering a low-cost, high-throughput method for single-cell genomics. However, this method has been limited in its ability to capture complete RNA transcription information. Researchers at Cornell – led by Iwijn De Vlaminck...
Read More »SCOPE-Seq – a scalable technology for linking live cell imaging and single-cell RNA sequencing
Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and ...
Read More »TAC-seq technology enhances precision of analysis of clinical biomarkers
Estonian scientists have announced the invention of a genetic testing technology to analyse the number of clinical biomarkers at the single-molecule level, which enhances the sensitivity of tests in precision medicine and will make them more affordable in future...
Read More »New RNA sequencing strategy provides insight into microbiomes
Tools allow scientists to understand the activity of naturally occurring microbiomes in response to real-world conditions and diet Researchers from the University of Chicago have developed a...
Read More »B cells and IgE antibodies underlying food allergies characterized by single cell RNA-seq
The production of antibodies by B cells is an important component of adaptive immunity. While antibodies often afford protection against pathogens such as viruses and bacteria, one class of antibodies, known as IgE, is involved in the pathogenesis of allergies ...
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