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A prerequisite to defining the transcriptome-wide functions of RNA modifications is the ability to accurately determine their location. NCI researchers have developed N4-acetylcytidine (ac4C) sequencing (ac4C-seq), a protocol for the quantitative single...

Terminal nucleotidyl transferases are enzymes that add non-templated nucleotides to RNA molecules. In the case of microRNAs, this process was shown to be functionally relevant for their maturation process...

In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to the COVID-19 pandemic, was reported. In the face of...

Alternative RNA isoforms are defined by promoter choice, alternative splicing, and polyA site selection. Although differential isoform expression is known to play a large regulatory role in eukaryotes, it has proved challenging to study with standard short-read RNA-seq because of ...

Conventional scRNA-seq expression analyses rely on the availability of a high quality genome annotation. Yet, as researchers show here with scRNA-seq experiments and analyses spanning human, mouse...

Glial cells of the central nervous system (CNS), including astrocytes and microglia, play critical roles in development, tissue repair, and homeostasis. However, dysregulated astrocyte and microglia...

Not all cancer cells within a tumor are created equal; nor do all immune cells (or all liver or brain cells) in your body have the same job. Much of their function depends on their location. Now, researchers at...

Next-generation sequencing allows researchers to efficiently determine the sequences of hundreds of millions of short DNA fragments from an experiment. Many experiments use next-generation sequencing...

Using a new single-cell technique, WEHI researchers have uncovered a way to understand the programming behind how stem cells make particular cell types. The research uncovered 30 new genes that program stem cells to make the dendritic cells that...

Single-cell measurements of cellular characteristics have been instrumental in understanding the heterogeneous pathways that drive differentiation, cellular responses to signals, and human disease...