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In small RNA (smRNA) sequencing studies, highly abundant molecules such as adapter dimer products and tissue-specific microRNAs (miRNAs) inhibit accurate quantification of lowly expressed species. Researchers from the HudsonAlpha Institute for Biotechnology previously developed a method to selectively deplete highly ...

Long INterspersed Elements-1 (LINEs/L1s) are repetitive elements that can copy and randomly insert in the genome resulting in genomic instability and mutagenesis. Understanding the expression patterns of L1 loci at the individual level will lend...

ANN ARBOR—A new way to cleanly separate out cancer cells from a blood sample enables comprehensive genetic profiling of the cancer cells, which could help doctors target tumors and monitor treatments more effectively. It is a dramatic improvement over current ...

Advances in RNA-sequencing methods have uncovered many aspects of RNA metabolism but are limited to surveying either the 3′ or 5′ terminus of RNAs, thus missing mechanistic aspects that ...

The study of pre-mRNA splicing has been greatly aided by the advent of RNA sequencing (RNA-seq), which enables the genome-wide detection of discrete splice isoforms. Quantification of these splice isoforms requires analysis of splicing informative...

The development of high-throughput single-cell RNA sequencing (scRNA-seq) has enabled access to information about gene expression in individual cells and insights into new biological areas. Although the interest in scRNA-seq has rapidly grown in recent...

Blood-based diagnostics tests, using individual or panels of biomarkers, may revolutionize disease diagnostics and enable minimally invasive therapy monitoring. However, selection of the most relevant biomarkers from liquid biosources remains an immense challenge. Researchers at the VU University Medical Center ...

EPFL bioengineers have developed a new method for Bulk RNA Sequencing that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq workflow with the performance of a bulk RNA-seq procedure. RNA sequencing is a technique used to analyze entire genomes by looking ...

A new technique called ECCITE-seq, developed by scientists at the New York Genome Center’s (NYGC) Technology Innovation Lab (@NYGCtech), allows researchers to perform high-throughput measurements of multiple modalities of information from single cells. The technique, ECCITE-seq, published today in Nature Methods, stands ...

Time series single-cell RNA sequencing (scRNA-seq) data are emerging. However, the analysis of time series scRNA-seq data could be compromised by 1) distortion created by assorted sources of data collection and generation across time samples...