Neuronal pathways recruit large postsynaptic populations and maintain connections via distinct postsynaptic response types (PRTs). Until recently, PRTs were only accessible as a selection criterion for single-cell RNA-sequencing (scRNA-seq) through probing by low-throughput whole-cell electrophysiology. To overcome these limitations and ...
Read More »Fragment-Seq – local tissue microenvironments at single-cell resolution
Cells collectively determine biological functions by communicating with each other-both through direct physical contact and secreted factors...
Read More »snHH-Seq – pan-cancer single-nucleus total RNA sequencing
Tumor heterogeneity and its drivers impair tumor progression and cancer therapy. Single-cell RNA sequencing is used to investigate the heterogeneity of tumor ecosystems. However, most methods of...
Read More »INVADEseq – cataloging bacterial diversity within tumor samples
It is said in Spanish, “si no existe, créalo” or “if it does not exist, create it.” This is exactly what sometimes occurs at the Fred Hutch. When we do not have the tools to test a hypothesis, we must ...
Read More »dSort-Seq – deep-learning–assisted high-throughput profiling of gene expression characteristics with high precision
Owing to the nondeterministic and nonlinear nature of gene expression, the steady-state intracellular protein abundance of a clonal population forms a distribution. The characteristics of this distribution...
Read More »COMPLETE-seq – profiling of repetitive RNA sequences in the blood plasma of patients with cancer
Liquid biopsies provide a means for the profiling of cell-free RNAs secreted by cells throughout the body. Although well-annotated coding and non-coding transcripts in blood are readily detectable and can serve as biomarkers of disease, the overall diagnostic utility of ...
Read More »CAP – a high-efficiency capture-based NGS approach for comprehensive analysis of mitochondrial transcriptome
The transcription of the mitochondrial genome is pivotal for maintenance of mitochondrial functions, and the deregulated mitochondrial transcriptome contributes to various pathological changes. Despite...
Read More »PRO-IP-seq – tracks molecular modifications of engaged Pol II complexes at nucleotide resolution
RNA Polymerase II (Pol II) is a multi-subunit complex that undergoes covalent modifications as transcription proceeds through genes and...
Read More »DAESC – differential allelic expression using single-cell data
Differential allele-specific expression (ASE) is a powerful tool to study context-specific cis-regulation of gene expression. Such effects can reflect the interaction between genetic or epigenetic factors...
Read More »New technique could help make precision medicine more widely available
Simple CUT&Tag adaptation gives information about genetic regulatory elements in formalin-fixed tumor samples. Tumor samples usually get soaked in formalin and then embedded in paraffin wax, ready for a pathologist to examine under a microscope...
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