Large RNA including mRNA (mRNA) has emerged as an important new class of therapeutics. Recently, this has been demonstrated by two highly efficacious vaccines based on mRNA sequences encoding for a modified version of the SARS-CoV-2 spike protein. There is ...
Read More »A LAMP sequencing approach for high-throughput co-detection of SARS-CoV-2 and influenza virus in human saliva
The COVID-19 pandemic has created an urgent need for rapid, effective, and low-cost SARS-CoV-2 diagnostic testing. Researchers at the University of Pennsylvania Perelman School of Medicine describe...
Read More »IMGG – integrating multiple single-cell datasets through connected graphs and generative adversarial networks
There is a strong need to eliminate batch-specific differences when integrating single-cell RNA-sequencing (scRNA-seq) datasets generated under different experimental conditions for downstream...
Read More »Halo-seq – An RNA proximity labeling method for the isolation and analysis of subcellular RNA populations
The subcellular localization of specific RNA molecules promotes localized cellular activity across a variety of species and cell types. The misregulation of this RNA targeting can result in developmental defects, and mutations in proteins that regulate this process are associated ...
Read More »NEAT-seq – simultaneous profiling of intra-nuclear proteins, chromatin accessibility and gene expression in single cells
Stanford University School of Medicine researchers have developed NEAT-seq (sequencing of nuclear protein epitope abundance, chromatin accessibility and the transcriptome in single cells), enabling...
Read More »An optimized skin tissue dissociation protocol for single-cell RNA sequencing analysis
Researchers from the University of Zurich present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell...
Read More »RiboRid – A low cost, advanced, and ultra-efficient method to remove ribosomal RNA for bacterial transcriptomics
RNA sequencing techniques have enabled the systematic elucidation of gene expression (RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq), and post...
Read More »PADME-seq reveals an increase in terminally exhausted T cells in the quiescent cancer cells niche
This study adapts a single-cell RNA-sequencing (RNA-seq) approach with precise spatial resolution to identify micro-niches of non-proliferating cancer cells within triple-negative breast cancer (TNBC) that function as immunotherapy-resistant reservoirs...
Read More »Micro-dissection and integration of long and short reads for identifying isoforms at microscopic structure-level
Studying isoform expression at the microscopic level has always been a challenging task. A classical example is kidney, where glomerular and tubulo-interstitial compartments carry out drastically different...
Read More »RNA-seq tomography (Tomo-seq) method adapted to create a spatially resolved genome-wide transcriptional atlas of the mouse nose
The sense of smell helps us navigate the environment, but its molecular architecture and underlying logic remain understudied. The spatial location of odorant receptor genes (Olfrs) in the nose is thought to be independent of the structural diversity of...
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