RNA-Seq Blog Transcriptome Research & Industry News
The combination of single-cell RNA sequencing with CRISPR inhibition/activation provides a high-throughput approach to simultaneously study the...
Read More »Evaluating capture sequence performance for single-cell CRISPR activation experiments
Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip
Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. One of the...
Read More »SUGAR-seq – simultaneous detection of glycans, epitopes, and the transcriptome in single cells
Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Researchers from the Peter MacCallum Cancer Centre describe SUrface-protein Glycan And ...
Read More »Targeted RNA-Seq assay incorporating UMIs improves quantification of transcripts from FFPE tumor tissue samples
Researchers from MD Anderson Cancer Center set out to assess whether modifications to a customized targeted RNA sequencing (RNAseq) assay to include unique molecular identifiers (UMIs) that collapse...
Read More »Single-nuclei RNA-sequencing of plant tissues
Single-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, researchers from the Max Delbrück Center for Molecular Medicine...
Read More »scDASH: a rRNA depletion method for single-cell total RNA-seq
To enable the profiling of whole transcriptome at single-cell level, researchers from the Hong Kong University of Science and Technology (HKUST) developed a method to deplete rRNA in total RNA from single-cell RNA-seq libraries, termed scDASH (single-cell depletion of abundant ...
Read More »Expansion sequencing – Spatially precise in situ transcriptomics in intact biological systems
In situ sequencing of physically expanded specimens enables multiplexed mapping of RNAs at nanoscale, subcellular resolution throughout intact tissues...
Read More »ARPIR – automatic RNA-Seq pipelines with interactive report
RNA-Seq is an increasing used methodology to study either coding and non-coding RNA expression. There are many software tools available for each phase of the RNA-Seq analysis and each of them uses different algorithms. Furthermore, the analysis consists of several ...
Read More »Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells
Single-cell RNA sequencing in principle offers unique opportunities to improve the efficacy of contemporary T-cell based immunotherapy against cancer. The use of high-quality single-cell data will aid our incomplete understanding of molecular...
Read More »AIR-seq – metabolic RNA labeling for probing RNA dynamics in bacteria
Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes...
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