Technology

RNA-binding proteins (RBPs) have essential functions during germline and early embryo development. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance...

First study to show gene expression changes in divers with ‘the bends’ reveals key role of genes for inflammation and immunity Diving and hyperbaric...

Tissues are complex mixtures of different cell subtypes, and this diversity is increasingly characterized using high-throughput single cell analysis methods. However, these efforts are hindered...

Although RNA sequencing (RNA-seq) has become the most advanced technology for transcriptome analysis, it also confronts various challenges. As we all know, the workflow of RNA-seq is extremely complicated and it is easy to produce bias. This may damage the ...

Recently, accumulating evidence has supported that circular RNA (circRNA) plays important roles in tumorigenesis by regulating gene expression at transcriptional and post-transcriptional levels. Expression of circRNAs can be epigenetically silenced by DNA methylation; however, the underlying regulatory mechanisms of circRNAs ...

Findings offer clues to why some types of renal cell carcinoma respond to immunotherapy while others do not. Not all kidney cancers behave the same, with...

Relative transcript abundance has proven to be a valuable tool for understanding the function of genes in biological systems. For the differential analysis...

RNA-targeted In Situ Sequencing for high throughput, high multiplex subcellular RNA analysis in a variety of tissues...

Single cell RNA sequencing (scRNA-seq) has become an important method to identify cell types, delineate the trajectories of cell differentiation in whole organisms and understand the heterogeneity in...

Single-cell RNA-seq (scRNA-seq) data simulation is critical for evaluating computational methods for analysing scRNA-seq data especially when ground truth is experimentally unattainable. The reliability of evaluation depends on the ability of simulation...