Presentations

NGS pipelines in UGENE

rna-seq

UGENE is a free open-source cross-platform bioinformatics software. It works perfectly on Windows, Mac OS and Linux and requires only a few clicks to install. This video shows a brief overview of NGS pipelines in UGENE: “Variant Calling with SAMtools”, ...

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RNA-Seq with EPIC-CoGe in 3 minutes

RNA-SEQ

This video tutorial shows how to clean and map RNA-Seq data to any genome in CoGe. This includes generating BAM files, quantitating reads per position across a genome, and generating FPKM for genes. These data can be kept private and ...

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RNA-Seq Shows its Sensitive Side

rna-seq

Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires more than 10 ng of RNA from 50–100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and ...

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