Sometimes, analyzing our DNA sequences isn’t quite enough. RNA-seq is a next-generation sequencing (NGS)-based technique that looks at the RNA transcripts produced from our DNA, allowing us to...
Read More »RNA sequencing and bioinformatics pipeline to identify expressed LINE-1s
Long INterspersed Elements-1 (LINEs/L1s) are repetitive elements that can copy and randomly insert in the genome resulting in genomic instability and mutagenesis. Understanding the expression patterns of L1 loci at the individual level will lend...
Read More »Multiparameter tissue section imaging and retrieval of image-defined micro-regions for RNA sequencing using the RareCyte platform
Tumor tissue imaging allows for a contextual understanding of tumor cells in relation to the immune microenvironment. The ability to interrogate tissues for multiple proteins that define microscopic regions of interest (ROI) and investigate gene expression...
Read More »Integrative Genomics Viewer – RNA-Seq Data Basics
In this Integrative Genomics Viewer (IGV) tutorial, we walk you through the basics of viewing RNA-Seq data in IGV.
Read More »Genetic analysis of differentiating cells using single-cell RNA-seq
This lecture was recorded at the ITN CONTRA workshop in Warsaw, Poland 2018. CONTRA (Computational ONcology TRaining Alliance) is an European Union funded Innovative Training...
Read More »RNA Sequencing – Case Studies & Clinical Relevance
Next-generation sequencing has evolved into a powerful diagnostic tool helping thousands get answers to the most challenging disease diagnoses. Even with today’s newest equipment, many...
Read More »MSI Tutorial – RNA Sequencing Analysis
Topic: RNA Sequencing Analysis Presented by: Tom Kono, Ph.D. Date: 11/6/18 Minnesota Supercomputing Institute
Read More »Differential Splicing Analysis with RNA-Seq: Current Applications, Approaches, & Limitations
Julian Egger PhD Student Department of Medical Informatics & Clinic Epidemiology
Read More »Bioconductor Workshop: RNA Seq and ChIP Seq Analysis
The Computational Biology Core (CBC) at Brown University (supported by the COBRE Center for Computational Biology of Human Disease) is teaming up with R/Bioconductor Staff to do two workshops...
Read More »RNA modifications poster – Discover the chemical structure of different RNA modifications and their distribution and function in mRNA and tRNAs
The field of epigenetics is branching down many new and exciting avenues. One of these avenues is the area of RNA modification research. Recent advancements in the development of RNA modification detection and sequencing methods...
Read More »