Publications

CellNet – network biology applied to stem cell engineering

rna-seq

Somatic cell reprogramming, directed differentiation of pluripotent stem cells, and direct conversions between differentiated cell lineages represent powerful approaches to engineer cells for research and regenerative medicine. A team led by researchers at Boston Children’s Hospital have developed CellNet, a ...

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High-Resolution Transcriptome Analysis with Long-Read RNA Sequencing

rna-seq

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput short-read sequencing of cDNA. However, as ongoing advances are rapidly yielding increasing ...

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Transcription Start Site Associated RNAs (TSSaRNAs) Are Ubiquitous in All Domains of Life

rna-seq

A plethora of non-coding RNAs has been discovered using high-resolution transcriptomics tools, indicating that transcriptional and post-transcriptional regulation is much more complex than previously appreciated. Small RNAs associated with transcription start sites of annotated coding regions (TSSaRNAs) are pervasive in ...

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The evolution of black widow venom

Black widow venom contains α-latrotoxin, infamous for causing intense pain. Combining 33kb of Latrodectus hesperus genomic DNA with RNA-Seq, we characterized the α-latrotoxin gene and discovered a paralog, 4.5kb downstream. Both paralogs exhibit venom gland specific transcription, and may be ...

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