Review Publications

High-throughput sequencing for the study of bacterial pathogen biology

rna-seq

A revolution in sequencing technologies in recent years has led to dramatically increased throughput and reduced cost of bacterial genome sequencing. An increasing number of applications of the new technologies are providing broad insights into bacterial evolution, epidemiology, and pathogenesis. ...

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Next-generation sequencing turns 10!

rna-seq

Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized ...

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Intracellular RNA-Seq

rna-seq

from Genetic Engineering News by Anton Simeonov, Ph.D. This literature review highlights a study led by George Church describing FISSEQ, or fluorescent in situ RNA sequencing. Methods such as fluorescence in situ hybridization (FISH) allow gene expression to be observed ...

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Single-cell RNA-seq: advances and future challenges

rna-seq

Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful ...

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Detecting and characterizing circular RNAs

rna-seq

Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and ...

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RNA Sequencing Methods

rna-seq

Technological advances in the sequencing field support in-depth characterization of the transcriptome. There are now many  genome-wide RNA sequencing methods used to investigate specific aspects of gene expression and its regulation, from transcription to RNA processing and translation: With the ...

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Cost comparison for qRT-PCR and RNA-Seq

rna-seq

Gene expression studies are widely diffused in life science research. So far, the most popular techniques adopted for transcript quantification are Northern blot, reverse transcription polymerase chain reaction (RT-PCR), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), microarrays, and RNA ...

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