RNA sequencing (RNA-Seq) and mass spectrometry-based shotgun proteomics are powerful high-throughput technologies for identifying and quantifying RNA transcripts and proteins respectively. With the increasing affordability of these technologies, many projects have started to apply both to the same samples to ...
Read More »Challenges and strategies for RNA-Seq data analysis
RNA-seq is a methodology for RNA profiling based on next-generation sequencing that enables to measure and compare gene expression patterns at unprecedented resolution. Although the appealing features of this technique have promoted its application to a wide panel of transcriptomics ...
Read More »RNA sequencing – current and prospective uses in metabolic research
Previous global RNA analysis was restricted to known transcripts in species with a defined transcriptome. Next generation sequencing has transformed transcriptomics by making it possible to analyse expressed genes with an exon level resolution from any tissue in any species ...
Read More »Bringing RNA-Seq closer to the clinic
Several multicenter benchmark data sets represent valuable steps toward using RNA-seq as a diagnostic tool with clinical utility. RNAs are excellent candidates for monitoring and diagnosing disease. In recent years, high-throughput RNA sequencing (RNA-seq) has opened new possibilities for determining ...
Read More »Application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes
Researchers at the Academy of Sciences of the Czech Republic propose ways to improve read mapping accuracy and sensitivity such as modifying a reference genome at RNA editing sites, using splicing- and ambiguity-competent aligners, and masking chloroplast- or nucleus-derived sequences. ...
Read More »International Review of Neurobiology – The Brain Transcriptome
The Brain Transcriptome International Review of Neurobiology – Volume 116, Pages 2-352 (2014) Chapter One – Introduction to Sequencing the Brain Transcriptome Pages 1-19 Robert Hitzemann, Priscila Darakjian, Nikki Walter, Ovidiu Dan Iancu, Robert Searles, Shannon McWeeney Chapter Two – ...
Read More »Incorporating alternative splicing and mRNA editing into the genetic analysis of complex traits
The nomination of candidate genes underlying complex traits is often focused on genetic variations that alter mRNA abundance or result in non-conservative changes in amino acids. Although inconspicuous in complex trait analysis, genetic variants that affect splicing or RNA editing ...
Read More »Introduction to RNA-Seq and its Applications to Drug Discovery and Development
The research and development (R&D) process in modern drug discovery and development is a challenging task. One of the reasons is because the biological system is complex. There are 20,000–25,000 genes in the human genome. Using traditional test methods such ...
Read More »High-throughput sequencing for the study of bacterial pathogen biology
A revolution in sequencing technologies in recent years has led to dramatically increased throughput and reduced cost of bacterial genome sequencing. An increasing number of applications of the new technologies are providing broad insights into bacterial evolution, epidemiology, and pathogenesis. ...
Read More »Next-generation sequencing turns 10!
Ten years ago next-generation sequencing (NGS) technologies appeared on the market. During the past decade, tremendous progress has been made in terms of speed, read length, and throughput, along with a sharp reduction in per-base cost. Together, these advances democratized ...
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