Review Publications

Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

rna-seq

Exosomes are tiny vesicles (30-150 nm) constantly secreted by all healthy and abnormal cells, and found in abundance in all body fluids. These vesicles, loaded with unique RNA and protein cargo, have a wide range of biological functions, including cell-to-cell ...

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Finding RNA Thermometers with Next-Gen Sequencing

Temperature is one of the decisive signals that a mammalian pathogen has entered its warm-blooded host. Among the many ways to register temperature changes, bacteria often use temperature-modulated structures in the untranslated region of mRNAs. In this article, the authors ...

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RNA-seq Data – Challenges in and Recommendations for Experimental Design and Analysis

rna-seq

RNA-seq is widely used to determine differential expression of genes or transcripts as well as identify novel transcripts, identify allele-specific expression, and precisely measure translation of transcripts. Thoughtful experimental design and choice of analysis tools are critical to ensure high-quality ...

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Leveraging the complementary nature of RNA-Seq and shotgun proteomics data

rna-seq

RNA sequencing (RNA-Seq) and mass spectrometry-based shotgun proteomics are powerful high-throughput technologies for identifying and quantifying RNA transcripts and proteins respectively. With the increasing affordability of these technologies, many projects have started to apply both to the same samples to ...

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