Challenges and Promises of RNA Diagnostics

Challenges and Promises of RNA Diagnostics
Air date: Wednesday, October 14, 2015, 3:00:00 PM
Category: WALS – Wednesday Afternoon Lectures
Runtime: 01:07:25
Description: Wednesday Afternoon Lecture Series

There is an increasing need in the life and medical sciences to determine gene sequence and gene expression in cells and tissues, including in pathogenic organisms and viruses under normal and disease conditions as well as organismal development. The limitations in diagnostic and prognostic tools have triggered interest in utilizing RNA present in extracellular fluid such as plasma and urine. RNA sequence analysis has the advantage of providing sequence as well as abundance information, and it also contains information such as the allelic sequence, mutations, or translocations, traditionally only mined by genomic DNA sequencing. There is reduced complexity at the RNA level, as tissues or cells never express simultaneously all human genes. Furthermore, important abundant transcripts encoded by multi copy genes or accompanied by pseudogenes are difficult to evaluate by genomic DNA sequencing yet are readily captured at the RNA level.

The Tuschl laboratory has developed RNA sequencing (RNAseq) approaches over many years and has discovered the genes encoding miRNAs and piRNAs. The group has revisited all classes of RNAs, including mRNAs, in order to arrive at a cleanly annotated and minimally redundant human transcriptome, including normal and disease-causing allelic variation, RNA editing sites, and isoforms. The emerging reference transcriptome is integrated in a large prototype database able to hold large experimental RNAseq data setsin order to supporting hierarchical annotation processes adaptable to any size of input RNA. This platform is able to generate in-depth reports of RNAabundance, specificity of expression across samples, detailed alignment reports and HTML displays, searching of reads and transcripts, and manually curating reference transcript entries. RNA reads without retrievable annotation are returned in form of assembled contigs to allow for discovery of novel genomic rearrangements, pathogens, or genes searching in public databases. Current software tools for alignment and mapping are fairly complicated and can only beused by domain experts, while they still miss to explain a substantial portion of RNAseq data. Yet recently, the Tuschl lab has developed automated approaches for isolating extracellular RNA from plasma/serum and urine samples and studied the small RNA composition by RNAseq in nearly 1,000 samples of normal subjectsand patients including liver, heart, and kidney diseases. The experimental as well as analytical challenges will be reviewed.

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Author: Thomas Tuschl, Ph.D., HHMI Investigator; Professor and Head of the Laboratory for RNA Molecular Biology; The Rockefeller University

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