A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.
RNA modifications poster – Discover the chemical structure of different RNA modifications and their distribution and function in mRNA and tRNAs
2 days ago
June 7, 2018
April 6, 2018