EMBL-EBI Train Online Course – RNA Sequencing

RNA sequencing (RNA-seq) is the application of next generation sequencing technologies to cDNA molecules. This is obtained by reverse transcription from RNA, in order to get information about the RNA content of a sample. Thus, RNA-seq is the set of experimental procedures that generates cDNA molecules derived from RNA molecules, followed by sequencing-library construction and massively parallel deep sequencing.

Advantages of RNA-seq over hybridisation-based approaches >>