Epitranscriptome Sequencing Technologies

An epitranscriptome is a set of functionally appropriate RNA modifications. There are nearly one hundred known modifications of RNA; the most common modification in internal mRNA is N6-methyladenosine (m6A). Transfer RNAs (tRNAs), mRNAs, and ribosomal RNAs (rRNAs) are the three types of RNAs that are post-transcriptionally modified.

In all living organisms,  translation is a fundamental process. The arrangement and operation of translation process is very expensive and it consumes 40% of cellular energy, which has led to the need for strict regulation of protein production in many aspects.

The regulation of translation is generally related to the need of regulated and non-regulated proteins. Nucleotide modifications are also important for the translation process. RNA base modifications are detected by appropriate sequencing methods.

(read more about the epitranscriptome sequencing technologies here…)

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