Featured RNA-Seq Job – Sr Genome Research Assistant

Case Western Reserve University (Cleveland, OH)

*Job Title:* Sr Genome Research Assistant
*Job ID:* 4934
*Department:* Epidemiology & Biostatistics
*Full/Part Time:* Full-Time
*Regular/Temporary:* Regular


Working with a high degree of independence and under the supervision of the principal investigator, the Senior Genome Research Assistant will design and perform highly multiplexed PCR experiments and single cell RNA-Seq using the Fluidigm Biomark platform and assist in analysis. The individual will also communicate directly with the principal investigator and collaborators to facilitate engagement of the principal investigator’s functional genomics laboratory in the completion of projects.


  1. Design, manage, perform and analyze single cell transcriptomes (e.g. RNA-Seq) and highly multiplexed PCR experiments with a high degree of independence.
  2. Design, validate and implement Fluidigm Biomark high-throughput PCR panels and apply Biomark technology to novel low-input (including single cell) protocols in next generation sequencing of the transcriptome.
  3. Provide supervision, technical guidance and training of technical staff developing novel low-input (including single cell) protocols in Fluidgim Biomark analysis of the human and macaque genome and transcriptome.
  4. Perform quality control, sequence alignment and preliminary data analysis of next generation sequencing and flow cytometry datasets.
  5. Contribute to research so as to merit serving as author or co-author on research publications.


  1. Maintain comprehensive inventory of samples, equipment and reagents and monitor lab budgets and expenses.
  2. Provide input and make recommendations to the principal investigator regarding significant developments in research projects within the lab and in the public domain.
  3. Perform other duties as assigned.


Department: Continuous contact with principal investigator and laboratory staff and postdocs.

University: Regular contact with purchasing, human resources, space and facilities planning, environmental health, safety/contact, Research Administration, IRB, and Sponsored Projects Accounting. Frequent contact with collaborators in other university departments and centers.

External: Regular contact with vendors and collaborators at other national and international universities, institutions and industry. Occasional contact with granting program directors and project managers.

Students: Moderate contact as needed with students.


Direct supervisory responsibility for laboratory technicians and research assistants. Direct supervisory responsibilities include the authority to screen, hire, assign, and evaluate laboratory employees or effectively recommend such actions to the principal investigator.


Experience: 5 years of academic lab experience with 3 years of experience in genomics, next generation sequencing and multiplex PCR technologies or related research.

Education: Master of Science degree in Molecular Biology, Microbiology, Immunology, Pharmacology, Bioinformatics, Biostatistics or a related discipline.


  1. Knowledge and understanding of commonly used concepts, practices, and procedures within the field of immunology, functional genomics, next generation sequencing and highly multiplexed PCR technologies.
  2. Ability to perform RNA/DNA isolation and amplification from human and animal samples.
  3. Ability to perform Fluidigm Biomark experiments with high quality control and assurance.
  4. Ability to perform flow cytometry analysis of subset and activation markers, with cell sorting experience.
  5. Demonstrated ability to perform preliminary sequencing and Fluidigm Biomark analysis preferred, including alignment and/or gene expression analysis.
  6. Familiarity with BSL-2+ policies and procedures and GCLP.
  7. Ability to develop and/or follow standard operating protocols to perform the functions of the job.
  8. A demonstrated peer-reviewed publication record is a strong advantage.
  9. Professional and effective oral and written communication skills; and good interpersonal skills with the ability to work and communicate with various individuals within and external to the university.
  10. Strong organization and independence skills; ability to multi-task, prioritize and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills, and follow-through.
  11. Effective academic and management skills; ability to lead, train, work with, and elicit cooperation from team members, staff and collaborators.
  12. Ability to work with sensitive information and maintain confidentiality.
  13. Effective problem-solving skills; must demonstrate excellent analytical skills, sound judgment and good decision-making.
  14. Must demonstrate flexibility and ability to work under pressure; must be able and willing to conform to shifting priorities, demands and timeline.
  15. Knowledge of basic accounting practices is preferred.
  16. Proficiency in Microsoft Office and database applications preferred.


Biosafety Level 2+ lab environment. Hazards include: electrical currents, airborne or contact exposure to bacterial or viral materials, human and animal blood/blood products/biological samples/tissues, toxic or caustic chemicals, and explosive gases.

Diversity Statement

In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

Reasonable Accommodations

Case Western Reserve University provides reasonable accommodations to applicants with disabilities. Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Inclusion, Diversity and Equal Opportunity at 216-368-8877 to request a reasonable accommodation. Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.


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