Featured RNA-Seq Job – Staff Research Associate I/II

The Staff Research Associate I/II (SRA I/II) will be working in a cell and molecular biology laboratory. The SRA I/II will be an integral member of a research team that is using CRISPR libraries in mammalian cell screens applied to a range of medically relevant topics, such as cancer. These will include screens performed by Perturb-seq, a platform for single-cell gene expression profiling of pooled CRISPR screens. Her/his responsibilities will include culturing, transducing, and maintaining human cell culture lines, performing flow cytometry, performing basic molecular biology techniques, performing basic statistical calculations, and other duties as assigned. Some time will be spent performing literature reviews and attending lab meetings/journal club meetings.Note that we are looking for either a SRA I or SRA II. The SRA I will be under direct supervision and training will be provided. Classification and salary will depend on the qualifications of the applicant.

The Department of Cellular and Molecular Pharmacology (CMP) within the UCSF School of Medicine pursues two related academic goals: [1] to discover new knowledge through innovative research on chemical and biological phenomena; [2] to transmit knowledge to students (graduate students, postdoctoral colleagues, and students and fellows in medicine, pharmacy and dentistry) at the conceptual and empirical levels.

About UCSF

The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences.

Required Qualifications

SRA I: BA/BS degree in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience

  • RNA-seq
  • Single-cell RNA-seq
  • Practical knowledge of basic molecular biology techniques and cell culture skills
  • Capability to learn and obtain a high- proficiency level with spinner flask cell cultures for a prolonged period of time (longer than a month in two liter culture)
  • Ability to effectively use/maintain laboratory instruments including flow cytometry machine
  • Excellent verbal and written communication skills
  • Ability to use internet as a resource for experimental tasks and literature searches
  • Capability to learn/use basic statistical tools

SRA II: In addition to the above, one or more years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position; or an equivalent combination of education and experience

Prefferred Qualifications

  • Bachelor’s degree in biology or chemistry
  • Familiarity with single cell RNA-seq
  • Knowledge of CRISPR/Cas9 system
  • Flow cytometry skills
  • Use of spinner flask for cell culture
  • For SRA II: In addition to the above, two or more years of experience

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