First microfluidics chip for creation of single cell libraries is commercialized

Dolomite, a world leader in microfluidics innovation, has launched the Single Cell RNA-Seq Chip for simple, reliable creation of single cell libraries. This new chip allows researchers to use droplet-based microfluidic methods to help explore the behaviour of thousands of individual cells, providing a rapid and easy way to screen for new or cryptic cell types.

The Single Cell RNA-Seq Chip – a commercially available version of the prototype PDMS Drop-­seq chip described by Macosko et al  – is manufactured from glass, offering excellent optical transparency and ensuring high precision. Combined with a very gentle sample loop method – to minimise bead shearing and contamination due to premature cell lysis – it enables rapid, straightforward preparation of single cell cDNA libraries. The chip offers robust and reproducible processing of 1 ml samples in approximately 12 hours, allowing cells to be individually encapsulated with uniquely barcoded RNA capture beads for high throughput sequencing.

For more information, visit the Single Cell RNA-Seq Chip webpage.

Source – Dolomite Microfluidics