Here, scientists at Academia Sinica, Taiwan describe the development of four methods of profiling mRNA 5′ ends using the Illumina sequencing platform;

  • The first method utilizes SMART (Switching Mechanism At 5′ end of RNA Transcript) technology.
  • The second involves replacing the 5′ cap structure with RNA oligomers via ligation.
  • The third and fourth methods are modifications of SMART, and involve enriching mRNA molecules with (nuclear transcripts) and without (mitochondrial transcripts) 5′ end cap structures, respectively.
rna-seq rna-seq
 rna-seq  rna-seq

 

Libraries prepared using SMART technology gave more reproducible results, but the ligation method was advantageous in that it only sequenced mRNAs with a cap structure at the 5′ end.

[box type=”shadow” align=”alignleft” ]Machida RJ, Lin Y-Y (2014) Four Methods of Preparing mRNA 5′ End Libraries Using the Illumina Sequencing Platform. PLoS ONE 9(7),  e101812. [article] [/fusion_text][/fusion_builder_column_inner][/fusion_builder_row_inner][/fusion_builder_column]

Related Posts

Here, scientists at Academia Sinica, Taiwan describe the development of four methods of profiling mRNA 5′ ends using the Illumina sequencing platform;

  • The first method utilizes SMART (Switching Mechanism At 5′ end of RNA Transcript) technology.
  • The second involves replacing the 5′ cap structure with RNA oligomers via ligation.
  • The third and fourth methods are modifications of SMART, and involve enriching mRNA molecules with (nuclear transcripts) and without (mitochondrial transcripts) 5′ end cap structures, respectively.
rna-seq rna-seq
 rna-seq  rna-seq

 

Libraries prepared using SMART technology gave more reproducible results, but the ligation method was advantageous in that it only sequenced mRNAs with a cap structure at the 5′ end.

[box type=”shadow” align=”alignleft” ]Machida RJ, Lin Y-Y (2014) Four Methods of Preparing mRNA 5′ End Libraries Using the Illumina Sequencing Platform. PLoS ONE 9(7),  e101812. [article] [/fusion_text][/fusion_builder_column_inner][/fusion_builder_row_inner][/fusion_builder_column]

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