Genetic screens – RNA-seq into the toolkit

ToolkitForward genetic screens in model organisms are powerful approaches for identifying mutations underlying various phenotypes and disease states. Methods to identify the causal mutation generally involve genetic mapping to a megabase-scale chromosomal region, followed by focal sequence analyses, but this can be laborious for organisms with complex genomes. Two new studies have devised pipelines based on high-throughput RNA sequencing (RNA-seq) for facilitating various aspects of forward genetic screens. (read more…)

  • Burgess DJ. (2013) Genetic screens: RNA-seq into the toolkit. Nat Rev Genet [Epub ahead of print]. [abstract]