In this webinar, researchers from NuGEN describe a novel method for targeted depletion of unwanted transcripts in RNA-Seq libraries. The proprietary Insert Dependent Adaptor Cleavage (InDA-C) technology employs customized probes to target specific transcript species for exclusion in the final RNA-Seq library. Unlike methods that use hybridization-mediated pull-down strategies to deplete unwanted RNA species prior to cDNA synthesis, the InDA-C method depletes targeted transcripts from RNA-Seq libraries, avoiding off-target mRNA cross-hybridization events that can potentially introduce bias.
Nanoscale Automation of a Full-Length RNA Sequencing Library Preparation Method on a Microfluidic Circuit
23 days ago
March 13, 2020
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