Hands-On Workshop – microRNA Analysis Using High-Throughput Sequencing


October 13th – 14th 2014

Leipzig, Germany

Scope and Topics

The purpose of this workshop is to obtain a thorough understanding on the expression profiling of known microRNAs and the prediction of novel microRNA genes using high-throughput sequencing technologies (HTS). The participants will be trained to understand both, the data analysis aspects of HTS technologies and the functional implications of small RNAs. Downstream analysis to infer the functional implication of the detected microRNAs will be discussed including target prediction/detection and functional analysis. After this workshop, the participants will be able to perform their own microRNA analysis using up-to-date methods and extract scientific valuable findings.

By the end of this workshop the participants will:

  • be familiar with the method of small RNA-Seq
  • be aware of the mapping problem of very short reads
  • understand common data formats and standards
  • know relevant tools for small RNA-Seq data processing
  • perform microRNA gene prediction and downstream analyses (target prediction, functional analysis, etc.)
  • plot and visualize results
  • be able to analyse small RNA-seq data


  • basic linux knowledge (shell usage, common commands). You should be familiar with the commands covered in the Learning the Shell Tutorial
  • basic understanding of molecular biology (DNA, RNA, gene expression, PCR, …)

Target Audience

  • biologists or data analysts with no or little experience in analyzing HTS data

Included in Course

  • Course materials
  • Catering
  • Conference Dinner


  1. Introduction to microRNAs
  2. Introduction to small RNA-Seq
  3. microRNA gene prediction
  4. Find differentially expressed microRNAs
  5. Perform microRNA target prediction
  6. Analysis of NGS target gene data, like PAR-CLIP or HITsCLIP
  7. Bring everything together and extract scientific valuable findings


Michael Hackenberg (University Granada) is the main developer of miRanalyzer, one of the most-used microRNA gene prediction tools using small RNA-seq data.

David Langenberger (ecSeq Bioinformatics) started working with small non-coding RNAs in 2006. Since 2009 he uses HTS technolgies to investigate these short regulatory RNAs as well as other targets. He analyzed small RNA-Seq data of several large projects, for example the International Cancer Genome Consortium (ICGC).

Key Dates

Opening Date of Registration: April 1st 2014
Closing Date of Early Registration: May 1st 2014
Closing Date of Registration: August 1st 2014
Workshop: October 13th-14th 2014 (8:00 – 17:00)


Location: Leipzig, Germany.
Language: English
Available seats: 20 (first-come, first-served)

Registration fees:

  • industry rate: 850 EUR (early registration: 750 EUR)
  • academic rate: 600 EUR (early registration: 500 EUR)

Travel expenses and accommodation are not covered by the registration fee.


ecSeq Bioinformatics
04275 Leipzig
Email: [email protected]

(find out more…)