Transcriptome analysis examines the complete set of RNA transcripts in a cell using high-throughput RNA-sequencing (RNA-seq). This valuable tool provides important information on gene function in a cell, a population of cells, or in an organism, that are under a specific set of conditions. RNA-seq identifies all RNA transcripts in a sample, including the regulatory miRNA and lncRNA transcripts. The sequences obtained by these methods can then be aligned to reference sequences to identify which genes have been transcribed in the sample.
Novogene is a leading genomics solution provider that offers a wide range of transcriptome sequencing services ranging from mRNA-sequencing to whole transcriptome analysis. Novogene’s services offer comprehensive solutions for the analysis of gene expression quantification and differential gene expression, the identification of novel transcripts, examining alternative splicing and gene fusion events, and more. The company’s bioinformaticians provide standard and customized data analysis, resulting in publication-ready results.
The experimental objective should dictate the type of sequencing that is used. For example, mRNA-sequencing ( mRNA-seq) focuses only on the coding RNAs, which make up approximately 2% of the entire transcriptome. This type of sequencing is ideal if research focuses only on these coding regions. mRNA-seq enables researchers to examine the variations in the cellular transcriptome by looking at the mRNA expression levels in a biological sample. It is often used to identify or compare gene expression levels between groups. Novogene’s mRNA-seq is performed on the Illumina NovaSeq platforms with paired-end 150 base pair sequencing read length. The sequencing depth varies based on whether a reference genome is available.
By comparison, whole transcriptome sequencing (WTS) looks at the set of genes that are transcribed by an organism at any given moment and includes all coding and non-coding RNA transcripts. This competitive approach is particularly useful for understanding what genes are being transcribed under different conditions. Once the transcriptome has been sequenced, the information can be used to ascertain which parts of the genome codes for active genes. This can be taken further by sequencing the transcriptome several times under different conditions or at different life stages to help understand which genes are involved in which biological processes. Novogene’s WTS service enables researchers to investigate potential transcriptional and regulatory network mechanisms by enabling an in-depth bioinformatics analysis on all transcripts including mRNAs and non-coding RNAs.
Aside from mRNA-seq and WTS, Novogene offers other transcriptome sequencing services including metatranscriptome analysis which examines the total content of gene transcripts in a community, such as soil, water, or in the gut, prokaryote RNA-sequencing, and an isoform-sequencing service that can be useful for detecting gene fusion events as well as providing more in-depth transcript annotation.
To improve the accuracy of their next-generation sequencing services further, Novogene launched a new product named Falcon in March 2020. Falcon is the first intelligent multi-product delivery platform for high-throughput NGS. Falcon promises to offer faster delivery with a more stable quality by eliminating human error and increasing efficiency. This new platform aims to increase the quality of Novogene’s services and meet the sequencing requirements of its customers.
The founder and CEO of Novogene, Dr Li Ruiqiang said, “Leveraging our years of expertise in genomic sequencing has enabled Novogene to develop and launch the first intelligent multi-product NGS delivery platform, which will provide a cutting-edge sequencing solution for customers. As a safe and accurate one-stop ground-breaking solution, Falcon enables faster delivery with more stable quality. As a leading provider of genomic services and solutions in the world, we want to be better positioned to drive innovation in the industry, to lead the industry in the direction towards becoming digital and automated, to propel the establishment of industry standards, and to eventually achieve intelligent transformation.”
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