Researchers at Stanford University show that RNA editing sites can be called with high confidence using RNA sequencing data from multiple samples across either individuals or species, without the need for matched genomic DNA sequence. They identified many previously unidentified editing sites in both humans and Drosophila; their results nearly double the known number of human protein recoding events. The researchers also found that human genes harboring conserved editing sites within Alu repeats are enriched for neuronal functions.
- Ramaswami G, Zhang R, Piskol R, Keegan LP, Deng P, O’Connell MA, Li JB. (2013) Identifying RNA editing sites using RNA sequencing data alone. Nat Methods 10(2), 128-32. [abstract]