In many biological applications, the readout of somatic mutations in individual cells is essential. For example, it can be used to mark individual cancer cells or identify progenies of a stem cell. Researchers from the Dana-Farber Cancer Institute present a protocol to perform single-cell RNA-seq and single-cell amplicon-seq using 10X Chromium technology. This protocol demonstrates how to (1) isolate CD34+ progenitor cells from human bone marrow aspirate, (2) prepare single-cell amplicon libraries, and (3) analyze the libraries to assign somatic mutations to individual cells.
Integrating readout of somatic mutations in individual cells with single-cell transcriptional profiling
Liu S, Nguyen M, Hormoz S. (2022) Integrating readout of somatic mutations in individual cells with single-cell transcriptional profiling. STAR Protoc 2(3):100673. [article]