Integrating readout of somatic mutations in individual cells with single-cell transcriptional profiling

In many biological applications, the readout of somatic mutations in individual cells is essential. For example, it can be used to mark individual cancer cells or identify progenies of a stem cell. Researchers from the Dana-Farber Cancer Institute present a protocol to perform single-cell RNA-seq and single-cell amplicon-seq using 10X Chromium technology. This protocol demonstrates how to (1) isolate CD34+ progenitor cells from human bone marrow aspirate, (2) prepare single-cell amplicon libraries, and (3) analyze the libraries to assign somatic mutations to individual cells.