from Genetic Engineering News by Anton Simeonov, Ph.D.
This literature review highlights a study led by George Church describing FISSEQ, or fluorescent in situ RNA sequencing.
Methods such as fluorescence in situ hybridization (FISH) allow gene expression to be observed at the tissue and cellular level; however, only a limited number of genes can be monitored in this manner, making transcriptome-wide studies impractical. George Church’s group* is presenting the further development of their original approach called fluorescent in situ sequencing (FISSEQ) to incorporate a spatially structured sequencing library and an imaging method capable of resolving the amplicons (see Figure 1).In fixed cells, RNA was reverse transcribed with tagged random hexamers to produce cDNA amplicons. Aminoallyl deoxyuridine 5-triphosphate (dUTP) was incorporated during reverse transcription and after the cDNA fragments were circularized before rolling circle amplification (RCA), an amine-reactive linker was used to cross-link the RCA amplicons containing aminoallyl dUTP. The team generated RNA sequencing libraries in different cell types, tissue sections, and whole-mount embryos for three-dimensional (3D) visualization that spanned multiple resolution scales (read more…)
from Genetic Engineering News by Anton Simeonov, Ph.D.
This literature review highlights a study led by George Church describing FISSEQ, or fluorescent in situ RNA sequencing.
Methods such as fluorescence in situ hybridization (FISH) allow gene expression to be observed at the tissue and cellular level; however, only a limited number of genes can be monitored in this manner, making transcriptome-wide studies impractical. George Church’s group* is presenting the further development of their original approach called fluorescent in situ sequencing (FISSEQ) to incorporate a spatially structured sequencing library and an imaging method capable of resolving the amplicons (see Figure 1).In fixed cells, RNA was reverse transcribed with tagged random hexamers to produce cDNA amplicons. Aminoallyl deoxyuridine 5-triphosphate (dUTP) was incorporated during reverse transcription and after the cDNA fragments were circularized before rolling circle amplification (RCA), an amine-reactive linker was used to cross-link the RCA amplicons containing aminoallyl dUTP. The team generated RNA sequencing libraries in different cell types, tissue sections, and whole-mount embryos for three-dimensional (3D) visualization that spanned multiple resolution scales (read more…)
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from Genetic Engineering News by Anton Simeonov, Ph.D.
This literature review highlights a study led by George Church describing FISSEQ, or fluorescent in situ RNA sequencing.
Methods such as fluorescence in situ hybridization (FISH) allow gene expression to be observed at the tissue and cellular level; however, only a limited number of genes can be monitored in this manner, making transcriptome-wide studies impractical. George Church’s group* is presenting the further development of their original approach called fluorescent in situ sequencing (FISSEQ) to incorporate a spatially structured sequencing library and an imaging method capable of resolving the amplicons (see Figure 1).In fixed cells, RNA was reverse transcribed with tagged random hexamers to produce cDNA amplicons. Aminoallyl deoxyuridine 5-triphosphate (dUTP) was incorporated during reverse transcription and after the cDNA fragments were circularized before rolling circle amplification (RCA), an amine-reactive linker was used to cross-link the RCA amplicons containing aminoallyl dUTP. The team generated RNA sequencing libraries in different cell types, tissue sections, and whole-mount embryos for three-dimensional (3D) visualization that spanned multiple resolution scales (read more…)
Related Posts
RNA Sequencing identifies new tick-borne virus that causes flu-like illness
Single-cell RNA sequencing links genetic mutations to cell behavior in cancer
Worm’s radical transformation shows metamorphosis can change the functions of cells
RNA sequencing reveals functional chimeric mRNAs in mammalian immunity
Atlas of the brain’s striatum could guide researchers to new drug treatments
Immune cells offer insights on billion-dollar virus
A functionally integrated cross-tissue alternative splicing program during short-term calorie restriction
Dietary oxidized plant sterol shifts macrophage state to fuel aortic inflammation
Machine learning approaches for biomarker discovery using single-cell RNA sequencing
From bench to bytes: a practical guide to RNA sequencing data analysis
Unlocking the past – new method helps gain insights into old tissue
Novel AI model trained on RNA-Seq data accurately detects key gene mutations and predicts biomarkers across 32 cancer types
Transcriptomic aging clock reveals age-related molecular patterns in opioid dependence
RNA sequencing helps predict stem cell transplant benefit in pediatric AML
Protein ‘switch’ determines whether liposarcoma cells will become aggressive
Precursor tRNAs sense temperature changes: heat stress-induced capped pre-tRNAs suppress protein synthesis
Ketamine increases neuroplasticity in female mice but not in males
Somatic mutations linked to vascular damage in progeria
Scientists map dormant cancer cells’ hideouts, opening new targets for treatment
Soluble signals released by neighboring cells direct how the human kidney is built
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