Iso-Seq – Full-length cDNA Sequencing of Alternatively Spliced Isoforms

rna-seq

The majority of human genes are alternatively spliced, making it possible for most genes to generate multiple proteins. Alternative splicing has been shown to play a role in human cancer, muscular dystrophy, Alzheimer’s, and many other diseases. Understanding these diseases requires knowing the full complement of mRNA isoforms.

Scientists at Pacific Biosciences have developed a technique, called Iso-Seq sequencing, that is capable of sequencing full-length, single-molecule cDNA sequences. The method employs SMRT Sequencing from PacBio, which can sequence individual molecules with read lengths that average more than 10 kb and can reach as long as 40 kb.

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