Pervasive transcription of the human genome generates RNAs whose mode of formation and functions are largely uncharacterized. Now researchers from Yale University School of Medicine have discovered yet another type of RNA: DoGs, which stands for – downstream of gene containing transcripts. They combined RNA-seq with detailed mechanistic studies to describe this transcript type derived from protein-coding genes. The resulting RNAs possess long non-coding regions (often >45 kb) and remain chromatin bound. DoGs are inducible by osmotic stress through an IP3 receptor signaling-dependent pathway, indicating active regulation. DoG levels are increased by decreased termination of the upstream transcript, a previously undescribed mechanism for rapid transcript induction. Relative depletion of polyA signals in DoG regions correlates with increased levels of DoGs after osmotic stress. The researchers detected DoG transcription in several human cell lines and provide evidence for thousands of DoGs genome wide.
Meet the latest RNAs – DoGs – downstream of gene containing transcripts
Vilborg, Passarelli MC, Yario TA, Tycowski KT, Steitz JA. (2015) Widespread Inducible Transcription Downstream of Human Genes. Mol Cell [Epub ahead of print]. [abstract]