Tumor tissue imaging allows for a contextual understanding of tumor cells in relation to the immune microenvironment. The ability to interrogate tissues for multiple proteins that define microscopic regions of interest (ROI) and investigate gene expression of cells in those regions is needed for advancing immuno-oncology therapeutic and biomarker discovery. Here we introduce Pick-Seq™, a novel method enabled by the RareCyte CyteFinder® Imaging System with integrated multiparameter imaging and micro-region retrieval capabilities for sequencing and transcript-level analysis.