Nanoscale Automation of a Full-Length RNA Sequencing Library Preparation Method on a Microfluidic Circuit

rna-seq

RNA sequencing (RNA-seq) has become the gold standard of expression profiling methods. We have developed an elegant microfluidics-based chemistry and workflow called the Advanta™ RNA-Seq NGS Library Prep Kit (PN 101-9187). Leveraging the Juno™ instrument, our RNA-Seq Kit supports simultaneous processing of up to 48 samples with a one-click script on our instrument.

The Juno NGS system automates the RNA-seq workflow along with a new, nanoscale integrated fluidic circuit (IFC) called 48.Atlas™, which is the size of a standard microtiter plate. The Advanta RNA-Seq NGS Library Prep Kit also includes reagents necessary to generate random-primed, stranded RNA-seq libraries from the polyadenylated RNA fraction present in as low as 10 ng of total RNA from eukaryotic organisms. Herein, we show comprehensive performance characteristics from several of our internal studies.

RNA-seq provides hypothesis-free profiling of transcript levels and isoforms. We have developed a highly cost-effective, nanoliter-volume microfluidics-based workflow and chemistry compatible with Illumina® sequencing instruments to simultaneously generate RNA-seq libraries from up to 48 samples. This method fully automates solid-phase capture of polyadenylated RNA, reverse transcription and index PCR within a compact nanoscale integrated fluidic circuit (IFC) on our Juno™ system. The workflow includes reagents necessary to generate random-primed RNA-seq libraries from as low as 10 ng of total RNA, while preserving strandedness information. In this poster we show comprehensive performance characteristics from our internal studies.

Source – Fluidigm

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