New: Zymo-Seq RiboFree™ Total RNA Library Prep Kit

Posted by: RNA-Seq Blog in Library Preparation, New Products, User Submitted Posts August 20, 2019 3,053 Views

Zymo Research announces early access to the first total RNA-Seq library prep kit with fully-integrated universal rRNA depletion.

Total RNA-Seq analysis enables researchers to analyze differential expression, novel transcripts, splicing and isoforms, SNP expression, and gene fusion and other mutation events, all from the same experiment. Current methods, however, are long (6.5-10.5 hrs), complex (>100 pipetting steps), require additional customization of specialized kits/modules for ribosomal RNA and/or other abundant transcript removal (e.g. Globin), which introduces bias into absolute transcript profiling. The Zymo-Seq RiboFree™ Total RNA Library Prep takes 3.5 hours to complete and works with any total RNA sample with little-to-no bias (Figure 1). It’s RNA-Seq, made simple.

The novel probe-free RiboFree™ Universal Depletion works across biological kingdom and phyla, including human, rodent, plant, and prokaryotes, as well as across a wide range of sample types including cells, tissue, FFPE tissues, and whole blood (Figure 2). Use one kit to deplete them all.

Zymo Research is constantly validating new sample types every day. Contact Zymo Research to try this kit with your sample!

Alternate probe-based methods such as TruSeq® Ribo-Zero™ Gold (Illumina®) deplete a significant number of non-target genes. Even greater bias is found in mRNA-Seq, as a result of poly(A) enrichment upstream of library preparation, leading to a distorted view of gene expression. RiboFree™ depletion uses only existing rRNA and Globin to target itself, leading to highly accurate transcriptome profiles (Figure 3). Make your libraries bias-free with probe-free RiboFree™ Universal Depletion.