To identify circulating miRNA biomarkers for AD, researchers from Meiji Pharmaceutical University, Japan reanalyzed a publicly available small RNA-Seq dataset, composed of blood samples derived from 48 AD patients and 22 normal control (NC) subjects, by a simple web-based miRNA data analysis pipeline that combines omiRas and DIANA miRPath.
By using omiRas, they identified 27 miRNAs expressed differentially between both groups. DIANA miRPath indicated that miRNA-regulated pathways potentially downregulated in AD are linked with neuronal synaptic functions, while those upregulated in AD are implicated in cell survival and cellular communication.
HCA of 27 miRNA s differentially expressed in blood of AD and NC. miRNA -Seq dataset of blood samples derived from 48 AD patients and 22 NC subjects was analyzed on omiRas.
The simple web-based miRNA data analysis pipeline helps to effortlessly identify candidates for miRNA biomarkers and pathways of AD from the complex small RNA-Seq data.
Availability – the omiRas web server is available at: http://tools.genxpro.net/omiras/