The purpose of this workshop is to introduce the methods of transcriptomic data generation and analysis at the level of single cells from diverse tissues. This workshop is supported by the Human Cell Atlas, an international project that aims to map every cell type in the human body, with the hope that this effort will transform our understanding of biology and disease and could lead to major advances in the way illnesses are diagnosed and treated. Although the methods that will be discussed in this workshop will be applicable to all organisms, the focus of this workshop will be on the human. The workshop will comprise live demonstration of experimental and data analysis methods. Members of the National Institute of Biomedical Genomics, India, and Massachusetts Institute of Technology, USA, will jointly teach in this online workshop. Members of the HCA will deliver special lectures.