Hilden, Germany, and Germantown, Maryland, November 12, 2019 – Last month QIAGEN announced the launch of innovative new technologies for faster, simpler library preparation for next-generation sequencing (NGS) in the growing field of RNA research. Ribonucleic acid (RNA) controls many ...
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New RNA sequencing strategy provides insight into microbiomes
Tools allow scientists to understand the activity of naturally occurring microbiomes in response to real-world conditions and diet Researchers from the University of Chicago have developed a...
Read More »Advantages of meta-total RNA sequencing (MeTRS) over shotgun metagenomics and amplicon-based sequencing in the profiling of complex microbial communities
Sequencing-based microbiome profiling aims at detecting and quantifying individual members of a microbial community in a culture-independent manner. While amplicon-based sequencing (ABS) of bacterial or...
Read More »Illumina launches its first semiconductor instrument for DNA, RNA sequencing
Illumina, Inc. announced today the launch of the iSeq™ 100 Sequencing System. The new, next-generation sequencing (NGS) system delivers exceptional data accuracy, at a low capital cost, making Illumina technology available to virtually...
Read More »A modified RNA-seq method for microbial community and diversity analysis using rRNA
RNA-seq-based SSU (small subunit) rRNA (ribosomal RNA) analysis has provided a better understanding of potentially active microbial community within environments. However, for RNA-seq library construction, high quantities of purified RNA are typically required. Fudan University researchers propose a modified RNA-seq ...
Read More »Norgen Biotek has launched a new Small RNA Library Prep Kit and new portfolio of RNA Sequencing Services
Norgen Biotek Corp., an innovative Canadian biotechnology company focusing primarily on advancing powerful tools for nucleic acid and protein purification and stabilization, today announced the recent launch of their new portfolio of product and service solutions for Next Generation Sequencing ...
Read More »Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
Global transcriptomic analysis via RNA-seq is often hampered by the high abundance of ribosomal (r)RNA in bacterial cells. To remove rRNA and enrich coding sequences, subtractive hybridization procedures have become the approach of choice prior to RNA-seq, with their efficiency ...
Read More »A fast and robust protocol for metataxonomic analysis using RNA-Seq data
Metagenomics is a rapidly emerging field aimed to analyze microbial diversity and dynamics by studying the genomic content of the microbiota. Metataxonomics tools analyze high-throughput sequencing data, primarily from 16S rRNA gene sequencing and DNAseq, to identify microorganisms and viruses ...
Read More »The Never-Ending Quest to Rewrite the Tree of Life
from Nova Next by Carrie Arnold – The bottom of the ocean is one of the most mysterious places on the planet, but microbiologist Karen Lloyd of the University of Tennessee, Knoxville, wanted to go deeper than that. In 2010, ...
Read More »scater – pre-processing, quality control, normalisation and visualisation of single-cell RNA-seq data in R
Single-cell RNA sequencing (scRNA-seq) is increasingly used to study gene expression at the level of individual cells. However, preparing raw sequence data for further analysis is not a straightforward process. Biases, artifacts, and other sources of unwanted variation are present ...
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