RNA-seq is a key technology for understanding the biology of the cell because of its ability to profile transcriptional and post-transcriptional regulation at single nucleotide resolutions. Compared to DNA sequencing alignment algorithms, RNA-seq alignment algorithms have a diminished ability to ...

The study of RNA has been dramatically improved by the introduction of Next Generation Sequencing platforms allowing massive and cheap sequencing of selected RNA fractions, also providing information on strand orientation (RNA-Seq). The complexity of transcriptomes and of their regulative ...

Transcript spike-in controls such as the ones devised by the External RNA Control Consortium (ERCC) have been successfully employed in RNA-Seq experiments to assess workflow and platform properties (Munroe et al., 2014). A limitation of these monocistronic, single-gene transcripts is ...

A well done RNA-Seq experiment can provide the most comprehensive, accurate and unbiased way to study gene expression, alternative splicing, RNA variation and RNA structure. The past few years have seen amazing technological advances that have led to a wide ...

The emergence of next-generation high-throughput RNA sequencing (RNA-Seq) provides tremendous opportunities for researchers to analyze alternative splicing on a genome-wide scale. However, accurate identification of alternative splicing events from RNA-Seq data has remained an unresolved challenge in next-generation sequencing (NGS) ...

May 13, 2015 Keynote: Next-Generation RNA-Seq Workflows and Analysis Gary Schroth,PhD Distinguished Scientist, Illumina, Inc. See Abstract A well done RNA-Seq experiment can provide the most comprehensive, accurate and unbiased way to study gene expression, alternative splicing, RNA variation and ...

[814.14] Simulation Studies Informed by RNA-seq Data Suggest the Utility of a Multi-network Bayesian Graphical Model Algorithm for the Study of Hypertension in the Dahl S Rat  Authors: Alex Dayton, Francesco Stingo, Chun Yang, Pengyuan Liu, Nadezhda N. Zheleznova, John D. ...

Transcriptome-wide RNA structure probing coupled with NGS yields the RNA structurome. RNA structurome data tremendously improve prediction of in vivo RNA structure. RNA structurome data uncover relationships between RNA structure and gene regulation. RNA folds into intricate structures that enable ...

RNA editing is a post-transcriptional/co-transcriptional molecular phenomenon whereby a genetic message is modified from the corresponding DNA template by means of substitutions, insertions, and/or deletions. It occurs in a variety of organisms and different cellular locations through evolutionally and biochemically ...

The emergence of next-generation RNA sequencing (RNA-Seq) provides tremendous opportunities for researchers to analyze alternative splicing on a genome-wide scale. However, accurate detection of intron retention (IR) events from RNA-Seq data has remained an unresolved challenge in next-generation sequencing (NGS) ...