RNA-Seq Blog

RNA-SEQ NEWS

viRome: an R package for the visualization and analysis of viral small RNA sequence datasets

May 30, 2013 Leave a comment 3,500 Views

RNA interference (RNAi) is known to play an important part in defense against viruses in a range of species. Second-generation sequencing technologies allow us to assay these systems and the ...

Investigating Evolutionary Perspective of Carcinogenesis with Single-cell Transcriptome Analysis

May 30, 2013 Leave a comment 1,614 Views

Researchers at USC have developed phase-switch microfluidic devices for molecular profiling of a large number of single cells. Whole genome microarrays and RNA-sequencing are commonly used to determine the expression ...

Presentation – Tools and Methods for RNA-seq Analysis

May 30, 2013 Leave a comment 1,569 Views

RNA-Seq Blog – Poll Results

May 29, 2013 Leave a comment 2,220 Views

We Asked – How long have you been involved with RNA Sequencing? Thanks to all who participated in the last poll. Check out our latest poll in the right hand ...

Ribosome profiling reveals resemblance between long non-coding RNAs and 5′ leaders of coding RNAs

May 29, 2013 Leave a comment 3,412 Views

Large-scale genomics and computational approaches have identified thousands of putative long non-coding RNAs (lncRNAs). It has been controversial, however, as to what fraction of these RNAs is truly non-coding. Here, ...

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Featured RNA-SEQ Events

RNA-Seq Summit 2018

January 12, 2018

Single Cell RNA Sequencing – Workshop

December 22, 2017

London Calling 2018

October 22, 2017

RNA 2018 Conference

September 6, 2017

Jobs

Featured RNA-Seq Job – Bioinformatics/Scientist RNA Biology (NCI)

6 days ago Leave a comment 723 Views

We are looking for a Bioinformatics/Scientist to provide dedicated bioinformatic support to the newly formed RNA Biology Laboratory in the Center for Cancer Research, National Cancer Institute. The RNA Biology Laboratory carries out cutting-edge...
Read More »
Featured RNA-Seq Job – Bioinformatics Scientist

13 days ago Leave a comment 1,032 Views
Postdoctoral fellowship in genetic epidemiology

19 days ago Leave a comment 466 Views
Featured RNA-Seq Job – Research Associate

20 days ago Leave a comment 543 Views
Open position for a biostatistician / computational biologist

27 days ago Leave a comment 1,510 Views

Industry News

Biostars RNA-Seq
SEQanswers - RNA Sequencing

Rna-Seq Pipeline

So, there're papers on designing an RNA-seq experiment, and normalizing the data (Bullard et. a [...]

Visualisation Of Alternative Splicing Events Using Rna-Seq Data

Hi, I am looking for tools that provide a nice graphical display of different alternative splicing e [...]

How Are Rna-Seq Transcripts Assigned?

I am looking at some RNA-seq data analyzed by cufflinks using standard conditions, and trying to det [...]

Tophat Time And Memory Requirement

Hi, Anyone has an idea of memory and time requirement for tophat 1.4.1 for 76 paired-end reads (~60M [...]

Rna-Seq Data Variant Calling

Has anyone tried calling variants from RNA-seq data and comparing those with WGS/Exome sequencing va [...]

Tutorial: Workshop - Analysis Of Next Generation Sequencing Data

Workshop - Analysis of Next Generation Sequencing Data (Programming for Evolutionary Biology 2012 ht [...]

Searching For A Simple Yet Powerful Workflow For Rna-Seq

Hi, I have to start working with RNA-Seq data and have worked previously with ChIP-Seq so I am famil [...]

Rna-Seq Data For Cd34+ Cells Or An Alternative Approach To Compare My Expression Set

Hi! I am looking for a RNA-seq data on CD34+ cells (human), to compare the expression of genes in my [...]

Distribution Of Isoform Length

Is there any paper that contains the distribution of isoform length in different organisms? Here I [...]

Building A Webpage For Accessing Rna-Seq Expression Data

Hello all, I would like to get some hints to go about this task. I'd like to design a webpage/s [...]

Tophat Error : Oserror: [Errno 2] No Such File Or Directory

Hi, I am running tophat and getting a lot of different errors, I could understand some of them, but [...]

Tutorial: Visualizing Chip-Seq Data Using Ucsc [Bigwig]

Hola! I would like to write a quick tutorial on viewing your coverage/track/wiggle files using UCSC. [...]

Why Are There Many Rna-Seq Hits To Intronic Regions?

I am looking at RNA-seq data, which I have little experience in. I notice that for many genes, there [...]

Differential Rnaseq Expression Analysis Directly On Stanford'S Great?

Does anybody know if the tool GREAT from Stanford U. can be used for RNAseq differential expression [...]

De Novo Transcriptome And Comparative Genomics

Hello all, First time posting here. I am looking at doing some comparative genomics with a mammalian [...]

How To Get Equal Numbers Of Gene_Id And Exon_Id For Use In Newexoncountset Function For Dexseq

I'm trying to run DEXseq but am having trouble with the newExonCountSet function because my gen [...]

Is This Always The Case That The Wig/Bigwig/Bed Files Are Meant Only For Viewing Into Ucsc Browser?

Since I'm currently working with RNA-seq experiment data stored in wig/bigwig format. There are [...]

Strand-Specific Rna-Seq Data For Maqc Samples?

Hi, Is there publicly available strand-specific RNA-Seq data for the MAQC samples? It seems that the [...]

Publicly Available Strand-Specific Rna-Seq Data With Corresponding Rt-Pcr Data?

Hi, I'm wondering if there publicly available strand-specific RNA-Seq data with corresponding R [...]

Public Rnaseq/Dnaseq Data

Hi, I understand this might be a long shot, but : is anybody aware of a public dataset which contain [...]

Advice for scRNA-seq Data

Hi, I am new to both scRNA-seq and this forum so hopefully this is posted in the correct place. I ha [...]

FeatureCounts high counts with strand 0,1,2 option

Dear featureCounts developers, I am working on RNASeq data which is generated using Truseq straded H [...]

Inconsistent RNAseq data in knockdown expt

Hi, I am helping a colleague to conduct a differential expression analysis with RNAseq data but I ha [...]

low GC% peak in one end of paired end reads

Hi, I have paired end RNA seq data prepared from Brassica napus using TruSeq kit. After adapter trim [...]

How to classify chIP-seq reads into gene bins?

Hello everyone! I am very new to RNA-seq data analysis. Your help on how to accomplish the below tas [...]

Evaluation of two main RNA-seq approaches for gene quantification in clinical RNA sequencing: polyA+ selection versus rRNA depletion

March 21, 2018