piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing

PIWI-interacting RNAs (piRNAs), 23-36 nt small silencing RNAs, repress transposon expression in the metazoan germ line, thereby protecting the genome. Although high-throughput sequencing has made it possible to examine the genome and transcriptome at unprecedented resolution, extracting useful information from gigabytes of sequencing data still requires substantial computational skills. Additionally, researchers may analyze and interpret the same data differently, generating results that are difficult to reconcile.

To address these issues, researchers at the University of Massachusetts Medical School developed a coordinated set of pipelines, ‘piPipes’, to analyze piRNA and transposon-derived RNAs from a variety of high-throughput sequencing libraries, including small RNA, RNA, degradome or 7-methyl guanosine cap analysis of gene expression (CAGE), chromatin immunoprecipitation (ChIP) and genomic DNA-seq. piPipes can also produce figures and tables suitable for publication. By facilitating data analysis, piPipes provides an opportunity to standardize computational methods in the piRNA field.

rna-seq

Gallery of piPipes Figures (A) Barplot representing length distribution of Drosophila w1 ovary small RNAs assigned to sense (blue) and antisense (red) strands of transposons. (B) Scatterplot comparing w1 to aubHN2/QC42 Drosophila ovary RNA-seq reads assigned to mRNA (NM; red), non-coding RNA (NR; green) and transposons (blue). (C) Metagene plot of H3K9me3 ChIP-seq of piRNA clusters from flies in which piwi mRNA was depleted by double-stranded RNA-triggered RNA driven by a triple Gal4 driver (SRX215630). (D) Circos plot representing the locations of, from the periphery to the center, cytological position, piRNA clusters, SV discovered by TEMP (tiles), retroSeq (tiles) and VariationHunter (links) using genomic sequencing of 2–4-day-old ovaries

Availability and implementation: piPipes is implemented in Bash, C++, Python, Perl and R. piPipes is free, open-source software distributed under the GPLv3 license and is available at http://bowhan.github.io/piPipes/

Contact: Phillip.Zamore@umassmed.edu or Zhiping.Weng@umassmed.edu

Han BW, Wang W, Zamore PD, Weng Z. (2014) piPipes: a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing. Bioinformatics [Epub ahead of print]. [article]

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