Post-doc position available – development of low-input high-throughput RNA-seq

Postdoctoral Research Scientist (Molecular Biology)

Applications are invited for a Postdoctoral Research Scientist (Molecular Biology) to join a collaborative project focussed on the development of low-input high-throughput RNA-seq at the Earlham Institute, based in Norwich, UK.


The project will adapt and enhance existing low-input RNA-seq approaches, led by Dr Iain Macaulay and Prof. Anthony Hall in collaboration with researchers from the Wilfried Haerty, Nicola Patron groups and the Earlham BioFoundry.

The role:

The role will involve the generation of an end-to-end workflow for low-input RNA-seq, from tissue sample through to sequence data. As part of this project, we will:

  • Develop optimised methods for RNA extraction from diverse tissue samples.
  • Build low-input RNA-seq libraries, applying modifications of the G&T-seq protocol (Macaulay et al. 2015) to directly lysed tissues and purified mRNA obtained from leaf punches.
  • Work with bioinformaticians to compare standard mRNA-seq sequence data with low-input RNA-seq data

The successful candidate will adapt and enhance existing low-input approaches, working with researchers from the Hall, Haerty and Macaulay groups and with the BioFoundry and Genomics Pipelines.

The candidate will develop skills in molecular biology, laboratory automation (e.g. using OpenTrons), high throughput sequencing and in building advanced molecular biology pipelines.

The ideal candidate:

Candidates should have a PhD in Molecular Biology or equivalent experience in high throughput sequencing and advanced molecular biology. Good communication and organisation skills with the ability to work independently or as part of multidisciplinary team are essential.

The position is initially for six months, although there maybe be opportunities to extend this.


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