From Biocompare by Anna Sowa
As a result of the large cost reduction for sequencing, RNA-seq is now a more affordable and popular technique in multiple areas, including clinical applications where the detection of aberrant transcription can be analyzed in human disease. And while each step of the experimental setup is of critical importance to get the most reliable results possible, and there are a number of ways to increase accuracy and success of RNA-seq experiments, sample prep stands at a pivotal point in experimental success.