quanTIseq – estimate the fractions of ten different immune cell types from RNA-Seq data

Researchers from Medical University of Innsbruck introduce quanTIseq, a method to quantify the fractions of ten immune cell types from bulk RNA-sequencing data. quanTIseq was extensively validated in blood and tumor samples using simulated, flow cytometry, and immunohistochemistry data. quanTIseq analysis of 8000 tumor samples revealed that cytotoxic T cell infiltration is more strongly associated with the activation of the CXCR3/CXCL9 axis than with mutational load and that deconvolution-based cell scores have prognostic value in several solid cancers. Finally, the researchers used quanTIseq to show how kinase inhibitors modulate the immune contexture and to reveal immune-cell types that underlie differential patients’ responses to checkpoint blockers.

quanTIseq method and validation based on blood-cell mixtures

rna-seq

a quanTIseq characterizes the immune contexture of human tumors from expression and imaging data. Cell fractions are estimated from expression data and then scaled to cell densities (cells/mm2) using total cell densities extracted from imaging data. b Heatmap of quanTIseq signature matrix, with z scores computed from log2(TPM+1) expression values of the signature genes. c The quanTIseq pipeline consists of three modules that perform (1) pre-processing of paired- or single-end RNA-seq reads in FASTQ format; (2) quantification of gene expression as transcripts-per-millions (TPM) and gene counts; and (3) deconvolution of cell fractions and scaling to cell densities considering total cells per mm2 derived from imaging data. The analysis can be initiated at any step. Optional files are shown in grey. Validation of quanTIseq with RNA-seq data from blood-derived immune cell mixtures generated in (d) and in this study (e). Deconvolution performance was assessed with Pearson’s correlation (r) and root-mean-square error (RMSE) using flow cytometry estimates as ground truth. The grey and blue lines represent the linear fit and the “x = y” line, respectively. B, B cells; CD4, non-regulatory CD4+ T cells; CD8, CD8+ T cells; DC, dendritic cells; M1, classically activated macrophages; M2, alternatively activated macrophages; Mono, monocytes; Neu, neutrophils; NK, natural killer cells; T, T cells; Treg, regulatory T cells

Availability – quanTIseq is available at http://icbi.at/quantiseq

Finotello F, Mayer C, Plattner C, Laschober G, Rieder D, Hackl H, Krogsdam A, Loncova Z, Posch W, Wilflingseder D, Sopper S, Ijsselsteijn M, Brouwer TP, Johnson D, Xu Y, Wang Y, Sanders ME, Estrada MV, Ericsson-Gonzalez P, Charoentong P, Balko J, de Miranda NFDCC, Trajanoski Z. (2019) Molecular and pharmacological modulators of the tumor immune contexture revealed by deconvolution of RNA-seq data. Genome Med 11(1):34. [article]

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