Quantitative miRNA profiling: how to choose the best RNA sequencing method?

From Proteintech by Dr. Karolina Szczesna

RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.

Why do we do RNA-seq?

RNA-seq has revolutionized approach to transcriptomics, it allows high-throughput being precise and cost-effective analysis of RNAs having advantages over microarrays and expressed sequence tag (EST) sequencing.

RNA-seq technology has many applications in basic and clinical research. It is mainly used for:

  • functional annotation of genomes by identifying all coding and non-coding RNAs, splice-sites, isoforms, open reading frames
  • quantitative analysis of RNA expression and expression profiling
  • detection of single nucleotide polymorphisms
  • studying RNA editing – RNA posttranscriptional modifications (PTMs)

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