Ribonucleic acid—RNA—gives scientists a way to analyze gene expression. Getting the best results, though, depends on analyzing the right RNA in an effective way. Increasingly, scientists select RNA sequencing (RNA-seq), but there’s more than one method for that. New tools and techniques allow scientists to use this approach in more scientific situations, from environmental science to medicine.
RNA-seq is not the only way to study DNA expression. That can also be accomplished with microarrays or quantitative polymerase chain reactions (qPCR), also called real-time PCR (RT-PCR). In comparing these methods, Brian Lilhanand, single cell multiomics leader for BD, pointed out several advantages of RNA-seq: uncovering novel transcripts, because the technique does not require species- or transcript-specific probes; larger dynamic range; better ability to detect rare transcripts; and easier multiplexing for the number of transcripts being analyzed.
(read more at Biocompare…)
Ribonucleic acid—RNA—gives scientists a way to analyze gene expression. Getting the best results, though, depends on analyzing the right RNA in an effective way. Increasingly, scientists select RNA sequencing (RNA-seq), but there’s more than one method for that. New tools and techniques allow scientists to use this approach in more scientific situations, from environmental science to medicine.
RNA-seq is not the only way to study DNA expression. That can also be accomplished with microarrays or quantitative polymerase chain reactions (qPCR), also called real-time PCR (RT-PCR). In comparing these methods, Brian Lilhanand, single cell multiomics leader for BD, pointed out several advantages of RNA-seq: uncovering novel transcripts, because the technique does not require species- or transcript-specific probes; larger dynamic range; better ability to detect rare transcripts; and easier multiplexing for the number of transcripts being analyzed.
(read more at Biocompare…)
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Ribonucleic acid—RNA—gives scientists a way to analyze gene expression. Getting the best results, though, depends on analyzing the right RNA in an effective way. Increasingly, scientists select RNA sequencing (RNA-seq), but there’s more than one method for that. New tools and techniques allow scientists to use this approach in more scientific situations, from environmental science to medicine.
RNA-seq is not the only way to study DNA expression. That can also be accomplished with microarrays or quantitative polymerase chain reactions (qPCR), also called real-time PCR (RT-PCR). In comparing these methods, Brian Lilhanand, single cell multiomics leader for BD, pointed out several advantages of RNA-seq: uncovering novel transcripts, because the technique does not require species- or transcript-specific probes; larger dynamic range; better ability to detect rare transcripts; and easier multiplexing for the number of transcripts being analyzed.
(read more at Biocompare…)
Related Posts
Worm’s radical transformation shows metamorphosis can change the functions of cells
RNA sequencing reveals functional chimeric mRNAs in mammalian immunity
Atlas of the brain’s striatum could guide researchers to new drug treatments
Immune cells offer insights on billion-dollar virus
A functionally integrated cross-tissue alternative splicing program during short-term calorie restriction
Dietary oxidized plant sterol shifts macrophage state to fuel aortic inflammation
Unlocking the past – new method helps gain insights into old tissue
Novel AI model trained on RNA-Seq data accurately detects key gene mutations and predicts biomarkers across 32 cancer types
Transcriptomic aging clock reveals age-related molecular patterns in opioid dependence
RNA sequencing helps predict stem cell transplant benefit in pediatric AML
Protein ‘switch’ determines whether liposarcoma cells will become aggressive
Precursor tRNAs sense temperature changes: heat stress-induced capped pre-tRNAs suppress protein synthesis
Ketamine increases neuroplasticity in female mice but not in males
Somatic mutations linked to vascular damage in progeria
Scientists map dormant cancer cells’ hideouts, opening new targets for treatment
Soluble signals released by neighboring cells direct how the human kidney is built
Genetics influence how cancer arises – and how it evolves
RNA-based testing uncovers extraordinary diversity in mutations driving lung cancer
Study offers new insights into why ex-smokers remain at elevated risk of lung disease
Learning the grammar of gene regulation
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