Revealing the Transcriptome with RNA-Seq

 Revealing the Transcriptome with RNA-Seq

Ribonucleic acid—RNA—gives scientists a way to analyze gene expression. Getting the best results, though, depends on analyzing the right RNA in an effective way. Increasingly, scientists select RNA sequencing (RNA-seq), but there’s more than one method for that. New tools and techniques allow scientists to use this approach in more scientific situations, from environmental science to medicine.

RNA-seq is not the only way to study DNA expression. That can also be accomplished with microarrays or quantitative polymerase chain reactions (qPCR), also called real-time PCR (RT-PCR). In comparing these methods, Brian Lilhanand, single cell multiomics leader for BD, pointed out several advantages of RNA-seq: uncovering novel transcripts, because the technique does not require species- or transcript-specific probes; larger dynamic range; better ability to detect rare transcripts; and easier multiplexing for the number of transcripts being analyzed.

(read more at Biocompare…)

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