This protocol presents a complete and detailed procedure to apply RNA-seq, a powerful next-generation DNA sequencing technology, to profile transcriptomes in human pulmonary microvascular endothelial cells with or without thrombin treatment. This protocol is generalizable to various cells or tissues affected by different reagents or disease states.
Cheranova D, Gibson M, Chaudhary S, Zhang LQ, Heruth DP, Grigoryev DN, Ye SQ. RNA-seq analysis of transcriptomes in thrombin-treated and control human pulmonary microvascular endothelial cells. J Vis Exp. 2013 Feb 13;(72). [abstract]