RNA-Seq in Space – Release of New Datasets by NASA GeneLab

Germinating Fern Spores Help Identify Genes Involved in Sensing and Responding to Gravity


A spore from the fern Ceratopteris richardii

During germination, a calcium current is triggered in the fern spore by a gravity sensing mechanism. The calcium current is part of a signalling cascade that orients the growth of the fern. Prior studies have determined that the critical timepoint for the gravity signal in the germination process occurs ten hours after the light signal that causes the onset of germination. In this study, transcriptional analysis was performed at ten hours post-germination to identify genes potentially important for the gravity response.

This study was supported by NASA Space Biology grants NNX09AH45G, NNX09AB41A, and NNX11AF48A to D. Marshall Porterfield and NSF grant IOS-1027514 to Stanley J. Roux

Study Title:         RNA-Seq analysis identifies potential modulators of gravity response in Ceratopteris spores: Evidence for modulation by calcium pumps and apyrase activity

Principal Investigator:    Stanley J. Roux, Ph.D.; University of Texas at Austin

Organism:           Ceratopteris richardii

Data Type:          RNA-seq reference transcriptome, Ceratoperis, data released by GeneLab

Study Link:          https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-57/

Bushart TJ, Cannon AE, Ul Haque A, San Miguel P, Mostajeran K, Clark GB, Porterfield DM, Roux SJ. (2016) RNA-seq analysis identifies potential modulators of gravity response in spores of Ceratopteris (Parkeriaceae): evidence for modulation by calcium pumps and apyrase activity. Am J Bot 100(1):161-74. [article]

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