RNA-seq in zebrafish models of obesity and anxiety show inverse regulation

Anxiety and metabolic impairments are often inter-related, but the underlying mechanisms are unknown. We subjected zebrafish larvae to caffeine-induced anxiety or high-fat diet-induced obesity followed by RNA sequencing and analyses. Differentially expressed  transcripts in both larval and adult models revealed inversely regulated DE transcripts. These included long noncoding RNAs and transfer RNA fragments, with overrepresented immune system and inflammation pathways.