RNA-Seq Blog Transcriptome Research & Industry News
RNA-SEQ – Mapping to a Reference Genome – by Kevin Libuit
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Thanks Kevin. I am new to bioinformatics and just beginning to understand the nuances of data analysis. Although I understand that TopHat aligns split-reads across splice junctions, I need to know which of the MANY aligners are actually better over the others and why?
Even though there might be many tools, I need to know why I should prefer to use a tool and not others. I hope you could shed some light on this difficult question. I work with mouse and human data and need to use spilce-junction reads for the analysis.
Thanks!