RNA transcription errors are transient, yet frequent, events that do have consequences for the cell. However, until recently we lacked the tools to empirically measure and study these errors. Advances in RNA library preparation and next generation sequencing (NGS) have allowed the spectrum of transcription errors to be empirically measured over the entire transcriptome and in nascent transcripts. Combining these powerful methods with forward and reverse genetic strategies has refined our understanding of transcription factors known to enhance RNA accuracy and will enable the discovery of new candidates. Furthermore, these approaches will shed additional light on the complex interplay between transcription fidelity and other DNA transactions, such as replication and repair, and explore a role for transcription errors in cellular evolution and disease.
Bradley CC, Gordon AJE, Halliday JA, Herman C. (2019) Transcription fidelity: New paradigms in epigenetic inheritance, genome instability and disease. DNA Repair (Amst). [Epub ahead of print]. [abstract]
Tagged with: Backtracked RNA polymerase DksA DNA break repair DNA resection Epimutation Genetic noise Genomic integrity GreA GreB Non-genetic phenotypic heterogeneity TFIIS Transcription error Transcription Factor Transcription fidelity