Technological advances in the sequencing field support in-depth characterization of the transcriptome. There are now many  genome-wide RNA sequencing methods used to investigate specific aspects of gene expression and its regulation, from transcription to RNA processing and translation:

[box type=”info” ]
  • tag-based methods for studying transcription
  • alternative initiation and polyadenylation events
  • shotgun methods for detection of alternative splicing
  • full-length RNA sequencing for the determination of complete transcript structures
  • targeted methods for studying the process of transcription and translation
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With the ensemble of technologies available, it is now possible to obtain a comprehensive view on transcriptome complexity and the regulation of transcript diversity.

rna-seq

[box type=”shadow” align=”alignleft” ]de Klerk E, den Dunnen JT, ‘t Hoen PA. (2014) RNA sequencing: from tag-based profiling to resolving complete transcript structure. Cell Mol Life Sci [Epub ahead of print]. [article][/fusion_text][/fusion_builder_column_inner][/fusion_builder_row_inner][/fusion_builder_column]

Technological advances in the sequencing field support in-depth characterization of the transcriptome. There are now many  genome-wide RNA sequencing methods used to investigate specific aspects of gene expression and its regulation, from transcription to RNA processing and translation:

[box type=”info” ]
  • tag-based methods for studying transcription
  • alternative initiation and polyadenylation events
  • shotgun methods for detection of alternative splicing
  • full-length RNA sequencing for the determination of complete transcript structures
  • targeted methods for studying the process of transcription and translation
[/fusion_text][/fusion_builder_column_inner][/fusion_builder_row_inner][/fusion_builder_column]

With the ensemble of technologies available, it is now possible to obtain a comprehensive view on transcriptome complexity and the regulation of transcript diversity.

rna-seq

[box type=”shadow” align=”alignleft” ]de Klerk E, den Dunnen JT, ‘t Hoen PA. (2014) RNA sequencing: from tag-based profiling to resolving complete transcript structure. Cell Mol Life Sci [Epub ahead of print]. [article][/fusion_text][/fusion_builder_column_inner][/fusion_builder_row_inner][/fusion_builder_column]

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